RPP10

Secretariat

EFB Team

Parc Cientific Barcelona
Baldiri Reixac 4-8 08028 Barcelona
.(JavaScript must be enabled to view this email address)
Phone: 00 34 93 4020599

Platinum sponsors

Gold Sponsors

Silver sponsor

Exhibitors

Prize awards:

25^th January, 2019 Abstract submission deadline
15^th February, 2019 Poster submission deadline
25^th January, 2019 Deadline for Early Bird Registration
15^th January, 2019 Bursary submission deadline
8^th March, 2019 Abstract decision notification
5^th April, 2019 Late registration deadline

Registration

The registration fee includes:

Meeting registration

Lunches and coffee breaks

Welcome cocktail - 24^th April

Dinner & conference banquet - 25^th & 26^th April

Register Now!

3 night’s accommodation (including breakfast) in conference hotel

Early Registration - before 25^th January, 2019

Young Researcher Registration

€ 550

Shared Room

Full Registration

€ 780

Full Registration - until 5^th April, 2019 (subject to capacity)

Young Researcher Registration

€ 595

Shared room

Full Registration

€ 880

Please note: If you have already registered and need to make changes to your registration or proceed with payment, please, log in by using your username and password.

Important information

Registration will only be accepted once full payment of registration fee has been received.

Deadline for submission of abstracts: 25^th January 2019

Deadline for submission of posters: 15^th February 2019

Call for Abstracts

The RPP2019 conference invites scientists from academia and industry to submit abstracts for oral and poster presentations describing original research in any of the following conference scientific topics:

Host physiology under RPP bioprocess conditions

Including gene expression and regulation, translation, protein folding and stability, secretion, stress responses and cellular quality control.

Cell engineering for RPP

Including systems metabolic engineering, synthetic biology tools, and integration of random and rational approaches.

RPP processes at industrial scale

Including challenges in up- and down-scaling, host physiology under bioprocess conditions, downstream processing, bioprocess analysis, product quality control, and the safety status of cell factories.

New-format and difficult to express proteins

Including multicomplex and multispecific proteins, membrane proteins, and cell-free technologies.

Deadline for abstract submission: January 25th , 2019

Deadline for poster submission: February 15th , 2019

Submit your abstract!

Guidelines and instructions

Please use the abstract form to prepare your submission:

Complete the registration form (you will not be required to pay immediately).
Follow the link to the abstract submission form.
Login by using the username and password employed at the registration form.
Follow the instructions and submit your abstract.

The abstract title should be less than 160 characters and must not be written in capital letters. Non-standard abbreviations should be avoided in titles, but may be used in the text if they are defined at first usage. The abstract should include the name of the presenting author underlined and affiliations of all authors. The body of the abstract should not exceed 1600 characters. Standard abbreviations and SI units must be used. Please ensure that your abstract does not include tables, charts or figures.

The scientific committee will select the type of contribution (oral or posters) based on the review of abstracts.

F.A.Q

Deadline for submission of abstracts:
25^th January, 2019

Deadline for submission of poster abstracts:
15^th February, 2019

You can submit one abstract.
If you need to edit the abstract you already submitted, please, send an email to .(JavaScript must be enabled to view this email address). Modifications will not be accepted after the 15^th February.

The corresponding author will receive an e-mail confirming the successful submission of the abstract. All abstracts for consideration as oral presentations will undergo peer review by the Scientific Committee. A decision on the acceptance of your abstract will be made as soon as possible.

Abstracts should be checked for accuracy prior to submission. Once the abstract has been submitted, changes, corrections or rewording will be possible until the abstract deadline on February 15th, 2019. After this date changes will not be possible. The presenting author is required to ensure that all co-authors are aware of the content of the abstract before submission. Abstract submission does not constitute registration for the conference. Abstract presenters must register to attend the conference by following the instructions for registration.

Submission of an abstract acknowledges your acceptance for the abstract to be published in the official RPP2019 Abstract Book.

Posters must be brought to the meeting by the presenting author and should not be mailed in advance. The required dimensions are 84 cm (width) by 120 cm (height) corresponding to an A0 format. At the top of a poster, please provide the title of the paper, names of the authors and their affiliations, all in bold lettering of about 2.5 to 3 cm size. Artwork and lettering should be large and clear enough so that the poster is readable from a distance of 1.5 m.

Important Submitting an abstract for presentation does not constitute registration for the conference. Abstract presenters must register to attend the conference by following the instructions for registration.

Creta Maris Meeting Room

Creta Maris Meeting Room-2

Creta Maris Meeting Room-3

Creta Maris Hotel Pool

Creta Maris dining room

Creta Maris - view from sky

How to get the venue

Island of Crete is served by international flights

Heraklion airport

Distance: 21kM

Time: 22 min

Taxi price: ~45€

Other options: more information soon.

Chania airport

Distance: 169kM

Time: 2 h 25 min

Taxi price: ~180€

Other options: more information soon.

Speakers

Nicole Borth Image

Nicole Borth

University of Natural Resources and Life Sciences & Austrian Centre of Industrial Biotechnology, Austria

Nathan Lewis Image

Nathan Lewis

University of California, USA

Francesca Ceroni Image

Francesca Ceroni

Imperial College London, UK

Tjeerd van Rij Image

Tjeerd van Rij

DSM Food Specialties, Netherlands

Tjeerd van Rij Image

Kerstin Otte

Biberach University of Applied Sciences, Germany

Andy Racher Image

Andy Racher

Lonza Pharma & Biotech, Switzerland

David Humphreys Image

Emma Davé

UCB Celltech, United Kingdom

Guido Grandi

Colin Robinson

University of Kent, UK

Diethard Mattanovich

University of Natural Resources and Life Sciences Vienna (BOKU), Austria

Jan Maarten van Dijl

University Medical Center Groningen (UMCG), Netherlands

Diethard Mattanovich

University of Natural Resources and Life Sciences Vienna (BOKU), Austria

Jan Maarten van Dijl

University Medical Center Groningen (UMCG), Netherlands

Cristina Bongiorni

DuPont, USA

Jeff Cole

University of Birmingham, UK

Alan Dickson

University of Manchester, UK

Pau Ferrer

Luxembourg Institute of Science and Technology (LIST), Luxembourg

Chris Finnis

University of Leicester, UK

Brigitte Gasser

Universität für Bodenkultur (BOKU), Austria

Stefan Hutwimmer

Sandoz, Austria

Joachim Klein

Lonza, Switzerland

Stefan Krahulec

Boehringer Ingelheim, Germany

Christine Lang

Technische Universität Berlin, Organobalance, Germany

Peter Neubauer

Technische Universität Berlin, Germany

Inga Norby

Novo Nordisk, Denmark

Kjeld Olesen

Novo Nordisk, Denmark

Tjeerd van Rij

DSM Food Specialties, Netherlands

Ursula Rinas

Helmholtz-Zentrum für Infektionsforschung (HZI), Germany

Markku Saloheimo

VTT, Finland

Joseph Shiloach

National Institutes of Health (NIH), USA

Ana Teixeira

Instituto de Biologia Experimental e Tecnológica (IBET), Portugal

Ken-ichi Yoshida

University of Kobe, Japan

Roland Weis

Validogen, Austria

Colin Robinson

University of Kent, UK.

Jan Maarten van Dijl

University Medical Center Groningen (UMCG), Netherlands

Diethard Mattanovich

University of Natural Resources and Life Sciences Vienna (BOKU), Austria

Ewa Wesolowska

European Federation of Biotechnology

Fernando Carreño

European Federation of Biotechnology

Ursula Rinas

Helmholtz Center Braunschweig, Germany

Name of the sessions (Subtopics):

Host physiology under RPP bioprocess conditions

Including gene expression and regulation, translation, protein folding and stability, secretion, stress responses and cellular quality control.

Cell engineering for RPP

Including systems metabolic engineering, synthetic biology tools, and integration of random and rational approaches.

RPP processes at industrial scale

New-format and difficult to express proteins

Including multicomplex and multispecific proteins, membrane proteins, and cell-free technologies.

Final Programme

Wednesday 24^th April

09:00 20:00	Registration

PreConference session: ProteinFactory & BioToP Open to all conference participants
10:30 10:40	Welcome & Introduction
10:40 10:55	Daphne Mermans Affiliation: University of Kent, UK and Stockholm University, Sweden. Title: Co-translational folding of proteins translocated by the Escherichia coli Twin Arginine pathway monitored in vivo and in vitro.
10:55 11:10	Margarita Bernal Cabas Affiliation: University of Groningen, the Netherlands Title: In vitro characterization of TatAyCy protein translocase complexes from Bacillus subtilis.
11:10 11:25	Minia Antelo Affiliation: University of Greifswald, Germany. Title: Absolute quantification of genome reduced Bacillus subtilis under secretion stress: A mass spectrometry-based proteomics approach.
11:25 11:40	Ana Bulovic Affiliation: Humboldt-Universität zu Berlin, Germany. Title: A whole-cell model of E. coli as an in silico experimental tool for recombinant protein production.
11:40 11:55	Sonakshi De Affiliation: BOKU, Austria. Title: Low Growth Rate triggered transition to pseudo-hyphal phenotype of the protein production host Pichia pastoris.
11:55 12:10	Dominik Jeschek Affiliation: BOKU, Austria. Title: A toolbox to express and characterize membrane transport proteins.
12:10 12:25	Philipp Mundsperger Affiliation: BOKU, Austria. Title: HIV-1 envelope glycoproteins as vaccine antigens - A critical reflection on expression and manufacture.
12:25 12:40	Nina Bydlinski Affiliation: BOKU, Austria. Title:Enhancement by reduction: Pushing N-glycosylation in CHO cells.
12:40 14:00	Lunch

Welcome & opening session C. Robinson & D. Mattanovich
14:00 14:20	Welcome & Introduction
14:20 14:50	Nathan Lewis Opening keynote lecture Affiliation: University of California, San Diego. Title: Unraveling the protein-specific requirements in mammalian protein expression.

Host physiology under recombinant protein production conditions Chair: Diethard Mattanovich
14:50 15:20	E. Tjeerd van Rij Affiliation: DSM, Netherlands. Title: Exploring synthetic expression modules with FACSseq in Bacillus subtilis.
15:20 15:40	Tobias Klein Affiliation: BASF, Germany. Title: The secretion stress response genes htrA and htrB are modulated by the choice of signal peptide during protein secretion in Bacillus.
15:40 16:00	Joseph Shiloach Affiliation: National Institutes for Health, USA. Title: Effect of over expressing protective antigen on global gene transcription in Bacillus anthracis BH500.
16:00 16:30	Coffee break
16:30 17:00	Francesca Ceroni Affiliation: Imperial College, UK. Title: The burden of recombinant protein production: a synthetic biology perspective.
17:00 17:20	Rosa Morra Affiliation: University of Manchester, UK. Title: Excretion of Cytoplasmic Proteins: are you missing out?.
17:20 17:40	Ken-ichi Yoshida Affiliation: Kobe University, Japan. Title: myo-Inositol-1-phosphate synthase restored in Bacillus subtilis to produce scyllo-inositol, a therapeutic agent for Alzheimer’s disease, from glucose.
17:40 18:00	Fabien Letisse Affiliation: LISBP Toulouse, France. Title: Dynamic metabolic response to heretologour protein overproduction in E.coli.
18:00 18:20	Ross Dalbey Affiliation: Ohio State University, USA Title: The mechanism of membrane insertion by the bacterial YidC.
Evening
	Poster setup
	Exhibition
	Welcome buffet

Thursday 25^th April

Cell engineering for recombinant protein production Chair:Colin Robinson
09:00 09:30	Kerstin Otte Affiliation: Biberach University of Applied Sciences, Germany Title: Engineering of mammalian cell lines to remove production bottlenecks.
09:30 09:50	Lise Marie Grav Affiliation: Novo Nordisk Foundation Center for Biosustainability, Denmark Title: Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation.
09:50 10:10	Ebru Alazi Affiliation: Dutch DNA Biotech BV, Netherlands Title: Rational design of fungal strains for production of plant biomass degrading enzymes.
10:10 10:30	Andreas Knapp Affiliation: Heinrich Heine University Düsseldorf, Germany Title: Small changes with big impact - how single nucleotide or amino acid substitutions affect production and secretion of recombinant proteins by Bacillus subtilis.
10:30 11:00	Coffee break
11:00 11:30	Patrick Stargardt Affiliation: enGenes Biotech, Germany Title: SGrowth-decoupled recombinant protein production in Escherichia coli.
11:30 11:50	Douglas Browning Affiliation: University of Birmingham, UK Title: The Rise of Garage Science: Making Recombinant Protein Production as Cheap as Chips.
11:50 12:10	Julie Zedler Affiliation: University of Copenhagen, Denmark Title: A fast-growing cyanobacterium: New hope for green cell factories.
12:10 12:30	Rocio Aguilar Suárez Affiliation: University Medical Center Groningen, The Netherlands Title: Going smaller: how “debugged” Bacillus Subtilis behaves as a cell factory.
12:30 13:30	Lunch
13:30 15:00	Poster Session:odd posters

Cell engineering for recombinant protein production Chair: Jan Maarten Van Dijil
15:00 15:20	Markku Saloheimo Affiliation: VTT Technical Research Center, Finland Title: Development of the filamentous fungus Myceliophtora Thermophila C1 into a next-generation therapeutic protein production system..
15:20 15:40	Jasmin Fischer Affiliation: Bisy, Austria Title: Inactivation of cell wall proteins to enhance protein secretion.
15:40 16:00	Alexander Frey Affiliation: Aalto University, Finland Title: Nature driven design of yeast based cell factories.
16:00 16:30	Coffee break
16:30 16:50	Andreas Licht Affiliation: Jena Biotech GmbH, Germany Title: Humanizing the glycosylation pattern of the Leishmania expression system LEXSY.
16:50 17:10	Lasse Pedersen Affiliation: Technical University, Denmark Title: CRISPR/Cas9 library screen >16000 gRNAs against CHO.
17:10 17:30	Gero Steinberg Affiliation: University of Exeter, UK Title: A cell biology approach towards understanding secretion in Trichoderma reesei.
17:30 18:10	Nicole Borth Keynote plenary lecture Affiliation: BOKU, Austria Title: Darwin and Lamarck unified: how to control phenotypes without changing the genome sequence.

Friday 26^th April

Recombinant protein production processes at industrial scale Chair: Pau Ferrer
09:00 09:30	Andy Racher Affiliation: Lonza, United Kingdom Title:To be confirmed.
09:30 09:50	Johanna Jarmer Affiliation: Boehringer Ingelheim RCV, Austria Title: Enhanced souble expression in Escherichia coli and miniaturized fed batch fermentation screening platform.
09:50 10:10	Núria Adelantado Affiliation: Lonza Pharma and Biotech, United Kingdom Title:XS™ Pichia 2.0: closing the gap between bacterial and mammalian systems for the production of recombinant proteins.
10:10 10:30	Peter Neubauer Affiliation: Technical University Berlin, Germany Title: Model based development of bioprocesses with recombinant microorganisms: Adaptive model based designs for efficient parameter estimation in parallel bioreactors.
10:30 11:00	Coffee break
11:00 11:20	Allan Kent Nielsen Affiliation: Novozymes, Denmark Title: Heterologous expression of amylase and PrsA pairs and their influence on the secretion stress response in Bacillus subtilis.
11:20 11:40	Roland Weis Affiliation: Validogen, Austria Title: Productivity through diversity - a protein production toolbox to UNLOCK Pichia.
11:40 12:00	David Wollborn Affiliation: RWTH Aachen, Germany Title: Small scale cultivation strategy ensuring the optimal induction time point for individual Komagataella phaffi clones during primary screening.
12:00 13:00	Lunch
13:00 15:00	Poster Session:even posters

New format and difficult to express proteins Chair: Jeff Cole
15:00 15:30	Emma Davé Affiliation: UCB Celltech, United Kingdom Title: To be confirmed.
15:30 15:50	Johanna Hausjell Affiliation: Technical University Viena, Austria Title: Challenge accepted: Recombinant production of hard-to-express membrane-bound cytochrome P450 in yeast .
15:50 16:10	Myrsini Michou Affiliation: National Hellenic Research Foundation, Greece Title: SuptoXD/SuptoxR: two specialized Escherichia coli strains for high-level recombinant membrane protein production .
16:10 16:40	Coffee break
16:40 17:00	Lloyd Ruddock Affiliation: University of Oulu, Finland Title: Sweet applications of disulfide bond formation in the cytoplasm.
17:00 17:20	Rochelle Aw Affiliation: Imperial College London, UK Title: Engineering a novel Pichia pastoris cell free protein synthesis platform.
17:20 17:50	Guido Grandi Affiliation: University of Trento, Italy Title: Synthetic Biology of Bacterial Outer Membrane Vesicles (OMVs) for the production of vaccines against infectious diseases and cancer.
Closing of the scientific programme. Concluding remarks
20:00	Conference dinner

Saturday 27^th April

Meeting of the EFB Microbial Physiology Section Board (upon invitation)
	Departure

The RPP10 registration includes the accommodation for the nights of the 24^th, 25^th and 26^th April on half board basis. If you would like to arrive earlier or leave later, you can contact the hotel (.(JavaScript must be enabled to view this email address)) and extend your booking, under special rates.

Double Room

garden view

140€

Half board

163€

All-inclusive

Single Room

garden view

130€

Half board

147€

All-inclusive

Family Room

open plan

210€

Half board

236€

All-inclusive

Creta Maris Beach - Image

Hotel
Creta Maris

Greece-Crete
700 14 Hersonissos

Novozymes Poster Prize Awards
The Novozymes poster awards are intended to recognize students demonstrating outstanding skills with regard to communicating new scientific and substantial results in the form of a poster. Prizes will be awarded to the best three posters presented by PhD students or young scientists in their first research position. If you qualify to enter this competition, please mark your poster with a label that will be provided by the Conference Organizers at the event. The first prize consists of a cash award in the amount of €1000. The second prize is a cash award of €500. The third prize is in the amount of €250.

The new edition on Recombinant Protein Production (RPP11) will take place in Girona, Spain from 17 – 19 October, 2023.

Please visit the website for more information: https://www.efbiotechnology.org/RPP

The RPP conference series aims at offering a comprehensive overview of current challenges and innovative solutions in recombinant protein production across different cell factories. Special attention is attributed to the comparative physiology of microbial and higher eukaryotic platforms for protein production. The 10th RPP conference will build on the successes of previous meetings, which have attracted steadily increasing numbers of researchers from both academia and the bioprocessing industry. The meeting will address four major areas and emerging trends in recombinant protein production of industrial relevance:

Host physiology under RPP bioprocess conditions

Including gene expression and regulation, translation, protein folding and stability, secretion, stress responses and cellular quality control.

Cell engineering for RPP

Including systems metabolic engineering, synthetic biology tools, and integration of random and rational approaches.

RPP processes at industrial scale

New-format and difficult to express proteins

Including multicomplex and multispecific proteins, membrane proteins, and cell-free technologies.

Platinum sponsors

Gold Sponsors

Silver sponsor

Organised by

Partner

Secretariat

Platinum sponsors

Gold Sponsors

Silver sponsor

Exhibitors

Prize awards:

Registration

The registration fee includes:

Call for Abstracts

Host physiology under RPP bioprocess conditions

Cell engineering for RPP

RPP processes at industrial scale

New-format and difficult to express proteins

Guidelines and instructions

F.A.Q

How to get the venue

Heraklion airport

Chania airport

Speakers

Nicole Borth

Nathan Lewis

Francesca Ceroni

Tjeerd van Rij

Kerstin Otte

Andy Racher

Emma Davé

Guido Grandi

Conference Chairs

Steering Group

EFB Organising Committee

Name of the sessions (Subtopics):

Host physiology under RPP bioprocess conditions

Cell engineering for RPP

RPP processes at industrial scale

New-format and difficult to express proteins

Final Programme

Wednesday 24th April

Daphne Mermans

Margarita Bernal Cabas

Minia Antelo

Ana Bulovic

Sonakshi De

Dominik Jeschek

Philipp Mundsperger

Nina Bydlinski

Nathan LewisOpening keynote lecture

E. Tjeerd van Rij

Tobias Klein

Joseph Shiloach

Francesca Ceroni

Rosa Morra

Ken-ichi Yoshida

Fabien Letisse

Ross Dalbey

Thursday 25th April

Kerstin Otte

Lise Marie Grav

Ebru Alazi

Andreas Knapp

Patrick Stargardt

Douglas Browning

Julie Zedler

Rocio Aguilar Suárez

Markku Saloheimo

Jasmin Fischer

Alexander Frey

Andreas Licht

Lasse Pedersen

Gero Steinberg

Nicole BorthKeynote plenary lecture

Friday 26th April

Andy Racher

Johanna Jarmer

Núria Adelantado

Peter Neubauer

Allan Kent Nielsen

Roland Weis

David Wollborn

Emma Davé

Johanna Hausjell

Wednesday 24^th April

Nathan Lewis
Opening keynote lecture

Thursday 25^th April

Nicole Borth
Keynote plenary lecture

Friday 26^th April

Saturday 27^th April

Hotel
Creta Maris